IECB
Seminars
Friday, March 4
11 am
@ IECB auditorium
Dr. Kevin Macé
Birkbeck college, Institute of Structural and Molecular Biology, UK
Type 4 secretion system cryo-EM structures
Bacteria Type 4 Secretion Systems (T4SS) are nanomachines that transfer substrates from a “donor” cell to a recipient “cell” via a long extracellular filament, termed pilus. T4SS have two main roles: i) used by prokaryotes to transfer DNA to another bacteria, a mechanism called “conjugation”; ii) used by some pathogenic bacteria to inject virulence proteins and DNA into pro- and eukaryotic cells. The conjugation is a major driver of genome dynamics and evolution in bacteria and plays a major role in the spread of antibiotic resistance and virulence genes among pathogens. Pathogenic bacteria use T4SS to induce serious diseases such as chronic infections and cancers. In this talk, I will present the first high-resolution structure of a 2.8 MegaDalton T4SS complex involved in pilus biogenesis obtained using cryo-EM and co-evolution analysis. Secondly, I will present the cryo-EM structure of pathogenic Legionella pneumophila substrate recruiting platform and its mechanism model.
Host: Rémi FRONZES