Friday, March 4

11 am

@ IECB auditorium

Dr. Kevin Macé

Birkbeck college, Institute of Structural and Molecular Biology, UK

Type 4 secretion system cryo-EM structures

Bacteria Type 4 Secretion Systems (T4SS) are nanomachines that transfer substrates from a “donor” cell to a recipient “cell” via a long extracellular filament, termed pilus. T4SS have two main roles: i) used by prokaryotes to transfer DNA to another bacteria, a mechanism called “conjugation”; ii) used by some pathogenic bacteria to inject virulence proteins and DNA into pro- and eukaryotic cells. The conjugation is a major driver of genome dynamics and evolution in bacteria and plays a major role in the spread of antibiotic resistance and virulence genes among pathogens. Pathogenic bacteria use T4SS to induce serious diseases such as chronic infections and cancers. In this talk, I will present the first high-resolution structure of a 2.8 MegaDalton T4SS complex involved in pilus biogenesis obtained using cryo-EM and co-evolution analysis. Secondly, I will present the cryo-EM structure of pathogenic Legionella pneumophila substrate recruiting platform and its mechanism model.

Host: Rémi FRONZES

IECB Friday seminars 2021

January 12, 2021:

Dr. Hervé Vezin (CNRS - Laboratoire Avancé de Spectroscopie pour les Interactions la Réactivité et l'Environnement (LASIRE), Université de Lille)

Title: Advanced EPR spectroscopy in material chemistry for batteries

February 5, 2021:

Dr. Hagen Hofmann (Department of Structural Biology, Weizmann Institute of Science)

Title: Allostery through DNA drives phenotype switching

March 5, 2021:

Prof. Camilo Perez (Biozentrum, University of Basel)

Title: Structure and mechanism of a proton dependent lipid transporter involved in lipoteichoic-acids biosynthesis

March 18, 2021:

Dr. Michael Eck (Dana-Farber Cancer Institute and Harvard Medical School)

Title: Insights into regulation of the Ras/Raf/MAPk pathway from Cryo-EM Structures of BRAF-MEK-14-3-3 Complexes

March 26, 2021:

Dr. Stephan Rauschenbach (University of Oxford)

Title: Electrospray ion beam deposition for single molecule imaging

April 2, 2021:

Prof. Bernard Rentier (Recteur Honoraire, Université de Liège)

Title: Open Science: Excellence revisited

April 15, 2021:

Dr. Nora Vazquez-Laslop (University of Illinois at Chicago)

Title: Macrolide antibiotics as modulators of translation

May 7, 2021:

Dr. Abhishek Chatterjee (Department of Chemistry, Boston College)

Title: Genetically encoded chemistries to read and write biology

May 28, 2021:

Dr. Julien Gronnier (Center for Plant Molecular Biology, University of Tübingen)

Title: Nanoscale regulation of cell-surface receptor signaling

June 3, 2021:

Dr. Jonathan Visentin (CHU de Bordeaux, Université de Bordeaux, Immuno ConcEpT, UMR CNRS 5164)

Title: Surface plasmon resonance to study anti-HLA antibodies: transfer of a basic science method to the bedside of organ transplant recipients and beyond

June 11, 2021:

Prof. Pierre Sonveaux & Prof. Raphaël Frédérick (Université catholique de Louvain)

Title: Discovery of an oxidative pathway of lactate in cancer and its druggability by inhibitors of lactate dehydrogenase 1 (LDH1) oligomerization

June 11, 2021:

Dr. Alexander Harms (Biozentrum, University of Basel)

Title: Drop out or go viral: A story of planned and unplanned expeditions into the phage world

June 25, 2021:

Dr. Eric Cornes (Institute Pasteur)

Title: Non-coding small RNAs as versatile regulators of germline gene expression programs

June 25, 2021:

Dr. Jacob Bobonis (EMBL Heidelberg)

Title: Bacterial retrons encode phage-sensing toxin/antitoxin systems

September 13, 2021:

Dr. Benoit Malleret (National University of Singapore)

Title: Molecular mechanisms of Plasmodium vivax invasion and cell tropism of zoonotic malaria species

September 22, 2021:

Prof. Valérie de Crécy-Lagard (University of Florida)

Title: Linking gene and function by comparative genomics: examples from deazapurine metabolism reveal cross-talks between RNA and DNA modifications

October 29, 2021:

Dr. Vishukumar Aimanianda (Institut Pasteur)

Title: Fungal cell-wall polysaccharides, exploiting their translational potential

November 26, 2021:

Dr. Niels Fischer (Max Planck Institute for Biophysical Chemistry, Germany)

Title: Single particle cryo-EM - Towards atomic-resolution of molecular machines in motion

Dr. Megan Wright (University of Leeds, UK)

Title: Chemical proteomic tools to study host-microbe interactions