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Tuesday, July 8
Tuesday, July 8
11:00 am
11:00 am
@ IECB auditorium
@ IECB auditorium
Prof. Michael SCHLIERF
Prof. Michael SCHLIERF
Technical University of Dresden, Germany
Technical University of Dresden, Germany
Molecular mechanisms of the bacterial integron revealed with single molecule biophysics
Molecular mechanisms of the bacterial integron revealed with single molecule biophysics
The predominant tool for adaptation in Gram-negative bacteria is a genetic system called integron. It rearranges gene cassettes, promoting multiple antibiotic resistances, a recognized major global health threat. It is based on a unique recombination process involving a Tyrosine recombinase - called integrase IntI - and folded single-stranded DNA hairpins - called attC sites. Hundreds of different attC sites can be recombined by IntI, yet it is still elusive how the recombinase recognizes the correct DNA hairpin and how the hairpin affects recombination efficiency. In this presentation, I will introduce how we use single-molecule FRET and single-molecule forces spectroscopy to study molecular mechanisms encoded in DNA sequence and proteins. I will discuss how different proteins compete for the identical single-stranded DNA and how naturally occurring sequences within the attC sites are optimized for genetic stability. I will also discuss how the attC sequence potentially regulates choice of DNA strand by slightly adjusted structures. We further developed an optical tweezers force-spectroscopy assay that allows us to probe the synaptic complex stability for different DNA substrates and protein variants. I will discuss, what DNA and protein elements regulate the stability of the macromolecular complex and how this could in turn regulate the recombination efficiency. With the acquired insights, we aim to design a component that would actively destabilize a given synaptic complex, by extension, inhibiting the spread of antibiotic resistances.
Host: Mikayel AZNAURYAN
Monday, December 5
Monday, December 5
14:00 am
14:00 am
@ IECB auditorium
@ IECB auditorium
Dr. Sébastien GOEB
Dr. Sébastien GOEB
Laboratory MOLTECH-Anjou, Angers University
Laboratory MOLTECH-Anjou, Angers University
Stimuli responsive coordination cages
Stimuli responsive coordination cages
Coordination driven self-assembly has allowed the preparation of many molecular polygons and polyhedrons with remarkable properties. They are obtained by association of polytopic ligands and complexes showing a pre-organized geometry. The corresponding host cavities offer promising opportunities for applications in molecular recognition or even in guest transport. We focused our attention for several years in the design of electro-active self-assembled 2D and 3D discrete structures based on the tetrathiafulvalene unit (TTF) and derivatives (exTTF and DTF for example) with the aim of controlling the guest release thanks to an electrochemical stimulus. We are also interested in understanding the key parameters governing the formation of an emergent class of coordination assemblies, i.e. interlocked cages. We believe that such compact objects could offer promising opportunities in controlling the structural organization of donor and acceptor units. For example, we demonstrated recently that truxene based ligands, associated with dinuclear Ruthenium or Rhodium complexes, can produce this type of compact interlocked systems.
Host: Yann FERRAND
Thursday, December 8
Thursday, December 8
11:15 am
11:15 am
@ IECB auditorium
@ IECB auditorium
Dr. Ana S. Ramirez
Dr. Ana S. Ramirez
Institute of Molecular Biology and Biophysics, ETH Zurich
Institute of Molecular Biology and Biophysics, ETH Zurich
Structural and mechanistic insight into protein N-glycosylation
Structural and mechanistic insight into protein N-glycosylation
Protein N-glycosylation is a post-translational modification that exists in all domains of life. It consists of two main steps: (1) biosynthesis of a lipid-linked oligosaccharide (LLO), and (2) the glycan transfer from LLO to asparagine residues of acceptor proteins, a process catalyzed by oligosaccharyltransferase (OST) enzymes. N-glycosylation plays essential roles in several cellular processes, such as protein folding, function, and cell-cell communication. During the last years, my research has focused on the structural and mechanistic investigation of N-glycosylation enzymes in bacteria, humans, yeast, and protozoan parasites using a multidisciplinary approach that involves protein biochemistry, chemo-enzymatic glycan elongation, X-ray crystallography, and single-particle Cryo-EM. We described a counting mechanism for PglH, a processive glycosyltransferase involved in LLO biosynthesis in the bacterial human pathogen Campylobacter jejuni [1]. In higher eukaryotes, where OST enzymes are multimeric membrane complexes located at the membrane of the endoplasmic reticulum (ER), we determined cryo-EM structures of the two OST enzymes that co-exist in human cells, revealing the molecular basis of their specific function in catalyzing co-translational or post-translational N-glycosylation [2]. Recently, we elucidated cryo-EM structures of the yeast multimeric OST complex in different functional states revealing the molecular basis of substrate recognition and catalysis [3].
1. Ramírez, A. S. et al. Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase. Nat. Commun. 9, 445 (2018).
2. Ramírez, A. S., Kowal, J. & Locher, K. P. Cryo-electron microscopy structures of human oligosaccharyltransferase complexes OST-A and OST-B. Science 366, 1372–1375 (2019).
3. Ramírez, A. S. et al. Molecular basis for glycan recognition and reaction priming of eukaryotic oligosaccharyltransferase. Nat. Commun. 13, 7296 (2022).
Host: Nicolas REYES
IECB Friday seminars 2022
IECB Friday seminars 2022
November 10, 2022:
November 10, 2022:
Prof. Ruchi Anand (Indian Institute of Technology Bombay)
Prof. Ruchi Anand (Indian Institute of Technology Bombay)
Title: Strategies to Combat Antibiotic Resistance
Title: Strategies to Combat Antibiotic Resistance
November 4, 2022:
November 4, 2022:
Dr. Gabriela Balíková Novotná (Institute of Microbiology of the Czech Academy of Sciences)
Dr. Gabriela Balíková Novotná (Institute of Microbiology of the Czech Academy of Sciences)
Title: ANTIBIOTIC RESISTANCE OR SIGNALING? The role of ABCF ATPases in antibiotic producers
Title: ANTIBIOTIC RESISTANCE OR SIGNALING? The role of ABCF ATPases in antibiotic producers
October 7, 2022:
October 7, 2022:
Dr. Chrystel Lopin-Bon (Institut de Chimie Organique et Analytique, Université d’Orléans)
Dr. Chrystel Lopin-Bon (Institut de Chimie Organique et Analytique, Université d’Orléans)
Title: Synthesis of Chondroitin Sulfate (CS) oligosaccharides as tools to decipher proteins-CS interaction
Title: Synthesis of Chondroitin Sulfate (CS) oligosaccharides as tools to decipher proteins-CS interaction
Dr. Vincent Ferrières (Institut des Sciences Chimiques de Rennes, ENSCR, Université de Rennes 1)
Dr. Vincent Ferrières (Institut des Sciences Chimiques de Rennes, ENSCR, Université de Rennes 1)
Title: Rare lichenic carbohydrates and advanced mass spectrometry
Title: Rare lichenic carbohydrates and advanced mass spectrometry
Dr. Carmen Galan (School of Chemistry, University of Bristol, UK)
Dr. Carmen Galan (School of Chemistry, University of Bristol, UK)
Title: Novel catalytic methods for the stereoselective synthesis of deoxyglycosides as diagnostic tools
Title: Novel catalytic methods for the stereoselective synthesis of deoxyglycosides as diagnostic tools
September 23, 2022:
September 23, 2022:
Dr. Emilie Moulin (Institut Charles Sadron (ICS), Strasbourg)
Dr. Emilie Moulin (Institut Charles Sadron (ICS), Strasbourg)
Title: Contractile Materials from Molecular Machines
Title: Contractile Materials from Molecular Machines
Dr. Julien Leclaire (Université Claude Bernard Lyon 1, Lyon)
Dr. Julien Leclaire (Université Claude Bernard Lyon 1, Lyon)
Title: Dynamic covalent chemistry at work: from the propagation of molecular information to the concomitant recycling of CO2 and metals from waste material
Title: Dynamic covalent chemistry at work: from the propagation of molecular information to the concomitant recycling of CO2 and metals from waste material
July 4, 2022:
July 4, 2022:
Dr. Tomohisa Sawada (Tokyo Institute of Technology, Japan)
Dr. Tomohisa Sawada (Tokyo Institute of Technology, Japan)
Title: Metal–peptide rings and nets with entangling topologies
Title: Metal–peptide rings and nets with entangling topologies
June 10, 2022:
June 10, 2022:
Dr. Leos Valasek (Institute of Microbiology of the Czech Academy of Sciences, Czech Republic)
Dr. Leos Valasek (Institute of Microbiology of the Czech Academy of Sciences, Czech Republic)
Title: Longer is not always the best; lessons from the tRNA anticodon stem
Title: Longer is not always the best; lessons from the tRNA anticodon stem
March 31, 2022:
March 31, 2022:
Dr. Fabienne Burlina (Laboratoire des Biomolécules, Sorbonne Université, Paris)
Dr. Fabienne Burlina (Laboratoire des Biomolécules, Sorbonne Université, Paris)
Title: Extending chemical ligation for the synthesis of proteins: application to NEMO ubiquitylation
Title: Extending chemical ligation for the synthesis of proteins: application to NEMO ubiquitylation
Dr. Yung-Sing Wong (Département de pharmacologie moléculaire, Equipe Chémobiologie et outils moléculaires thérapeutiques, Université Grenoble-Alpes, Grenoble)
Dr. Yung-Sing Wong (Département de pharmacologie moléculaire, Equipe Chémobiologie et outils moléculaires thérapeutiques, Université Grenoble-Alpes, Grenoble)
Title: Benefits of forming spatio-temporal chemical bonds in living environments
Title: Benefits of forming spatio-temporal chemical bonds in living environments
March 18, 2022:
March 18, 2022:
Dr. Kevin Macé (Birkbeck college, Institute of Structural and Molecular Biology, UK)
Dr. Kevin Macé (Birkbeck college, Institute of Structural and Molecular Biology, UK)
Title: Type 4 secretion system cryo-EM structures
Title: Type 4 secretion system cryo-EM structures
IECB Friday seminars 2021
IECB Friday seminars 2021
January 12, 2021:
January 12, 2021:
Dr. Hervé Vezin (CNRS - Laboratoire Avancé de Spectroscopie pour les Interactions la Réactivité et l'Environnement (LASIRE), Université de Lille)
Dr. Hervé Vezin (CNRS - Laboratoire Avancé de Spectroscopie pour les Interactions la Réactivité et l'Environnement (LASIRE), Université de Lille)
Title: Advanced EPR spectroscopy in material chemistry for batteries
Title: Advanced EPR spectroscopy in material chemistry for batteries
February 5, 2021:
February 5, 2021:
Dr. Hagen Hofmann (Department of Structural Biology, Weizmann Institute of Science)
Dr. Hagen Hofmann (Department of Structural Biology, Weizmann Institute of Science)
Title: Allostery through DNA drives phenotype switching
Title: Allostery through DNA drives phenotype switching
March 5, 2021:
March 5, 2021:
Prof. Camilo Perez (Biozentrum, University of Basel)
Prof. Camilo Perez (Biozentrum, University of Basel)
Title: Structure and mechanism of a proton dependent lipid transporter involved in lipoteichoic-acids biosynthesis
Title: Structure and mechanism of a proton dependent lipid transporter involved in lipoteichoic-acids biosynthesis
March 18, 2021:
March 18, 2021:
Dr. Michael Eck (Dana-Farber Cancer Institute and Harvard Medical School)
Dr. Michael Eck (Dana-Farber Cancer Institute and Harvard Medical School)
Title: Insights into regulation of the Ras/Raf/MAPk pathway from Cryo-EM Structures of BRAF-MEK-14-3-3 Complexes
Title: Insights into regulation of the Ras/Raf/MAPk pathway from Cryo-EM Structures of BRAF-MEK-14-3-3 Complexes
March 26, 2021:
March 26, 2021:
Dr. Stephan Rauschenbach (University of Oxford)
Dr. Stephan Rauschenbach (University of Oxford)
Title: Electrospray ion beam deposition for single molecule imaging
Title: Electrospray ion beam deposition for single molecule imaging
April 2, 2021:
April 2, 2021:
Prof. Bernard Rentier (Recteur Honoraire, Université de Liège)
Prof. Bernard Rentier (Recteur Honoraire, Université de Liège)
Title: Open Science: Excellence revisited
Title: Open Science: Excellence revisited
April 15, 2021:
April 15, 2021:
Dr. Nora Vazquez-Laslop (University of Illinois at Chicago)
Dr. Nora Vazquez-Laslop (University of Illinois at Chicago)
Title: Macrolide antibiotics as modulators of translation
Title: Macrolide antibiotics as modulators of translation
May 7, 2021:
May 7, 2021:
Dr. Abhishek Chatterjee (Department of Chemistry, Boston College)
Dr. Abhishek Chatterjee (Department of Chemistry, Boston College)
Title: Genetically encoded chemistries to read and write biology
Title: Genetically encoded chemistries to read and write biology
May 28, 2021:
May 28, 2021:
Dr. Julien Gronnier (Center for Plant Molecular Biology, University of Tübingen)
Dr. Julien Gronnier (Center for Plant Molecular Biology, University of Tübingen)
Title: Nanoscale regulation of cell-surface receptor signaling
Title: Nanoscale regulation of cell-surface receptor signaling
June 3, 2021:
June 3, 2021:
Dr. Jonathan Visentin (CHU de Bordeaux, Université de Bordeaux, Immuno ConcEpT, UMR CNRS 5164)
Dr. Jonathan Visentin (CHU de Bordeaux, Université de Bordeaux, Immuno ConcEpT, UMR CNRS 5164)
Title: Surface plasmon resonance to study anti-HLA antibodies: transfer of a basic science method to the bedside of organ transplant recipients and beyond
Title: Surface plasmon resonance to study anti-HLA antibodies: transfer of a basic science method to the bedside of organ transplant recipients and beyond
June 11, 2021:
June 11, 2021:
Prof. Pierre Sonveaux & Prof. Raphaël Frédérick (Université catholique de Louvain)
Prof. Pierre Sonveaux & Prof. Raphaël Frédérick (Université catholique de Louvain)
Title: Discovery of an oxidative pathway of lactate in cancer and its druggability by inhibitors of lactate dehydrogenase 1 (LDH1) oligomerization
Title: Discovery of an oxidative pathway of lactate in cancer and its druggability by inhibitors of lactate dehydrogenase 1 (LDH1) oligomerization
June 11, 2021:
June 11, 2021:
Dr. Alexander Harms (Biozentrum, University of Basel)
Dr. Alexander Harms (Biozentrum, University of Basel)
Title: Drop out or go viral: A story of planned and unplanned expeditions into the phage world
Title: Drop out or go viral: A story of planned and unplanned expeditions into the phage world
June 25, 2021:
June 25, 2021:
Dr. Eric Cornes (Institute Pasteur)
Dr. Eric Cornes (Institute Pasteur)
Title: Non-coding small RNAs as versatile regulators of germline gene expression programs
Title: Non-coding small RNAs as versatile regulators of germline gene expression programs
June 25, 2021:
June 25, 2021:
Dr. Jacob Bobonis (EMBL Heidelberg)
Dr. Jacob Bobonis (EMBL Heidelberg)
Title: Bacterial retrons encode phage-sensing toxin/antitoxin systems
Title: Bacterial retrons encode phage-sensing toxin/antitoxin systems
September 13, 2021:
September 13, 2021:
Dr. Benoit Malleret (National University of Singapore)
Dr. Benoit Malleret (National University of Singapore)
Title: Molecular mechanisms of Plasmodium vivax invasion and cell tropism of zoonotic malaria species
Title: Molecular mechanisms of Plasmodium vivax invasion and cell tropism of zoonotic malaria species
September 22, 2021:
September 22, 2021:
Prof. Valérie de Crécy-Lagard (University of Florida)
Prof. Valérie de Crécy-Lagard (University of Florida)
Title: Linking gene and function by comparative genomics: examples from deazapurine metabolism reveal cross-talks between RNA and DNA modifications
Title: Linking gene and function by comparative genomics: examples from deazapurine metabolism reveal cross-talks between RNA and DNA modifications
October 29, 2021:
October 29, 2021:
Dr. Vishukumar Aimanianda (Institut Pasteur)
Dr. Vishukumar Aimanianda (Institut Pasteur)
Title: Fungal cell-wall polysaccharides, exploiting their translational potential
Title: Fungal cell-wall polysaccharides, exploiting their translational potential
November 26, 2021:
November 26, 2021:
Dr. Niels Fischer (Max Planck Institute for Biophysical Chemistry, Germany)
Dr. Niels Fischer (Max Planck Institute for Biophysical Chemistry, Germany)
Title: Single particle cryo-EM - Towards atomic-resolution of molecular machines in motion
Title: Single particle cryo-EM - Towards atomic-resolution of molecular machines in motion
Dr. Megan Wright (University of Leeds, UK)
Dr. Megan Wright (University of Leeds, UK)
Title: Chemical proteomic tools to study host-microbe interactions
Title: Chemical proteomic tools to study host-microbe interactions
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